Oladipo I.C. , Ibrahim A.I., Ogunsona S.B. & Ogunleke O.B.
Vol 9, Issue 1, Article number: 34 (Mar, 2025)
Abstract
The focus of the present study was the characterization and biomedical properties of the silver nanoparticles (AgNPs) produced using the leaf extract of Ageratum conyzoides as a capping and reducing agent. The synthesized AgNPs were characterized by Ultraviolet Visible (UV-Vis) spectroscopy, Fourier transform infrared spectroscopy (FTIR), Energy Dispersive X-ray (EDX) and scanning electron microscopy (SEM). The in-vivo wound healing, antimicrobial, antioxidant, anticoagulant, and thrombolytic activities were evaluated. In wound healing assessment which was conducted on rats, the AgNPs was able to achieve a high wound closure of 96%. Furthermore, the nanoparticles also down regulated the total white blood cell counts in the treated rats which suggest that the rats were protected from infectious agents. The synthesized AgNPs exhibited significant activity in anticoagulation assay with 41.5, 81.5 and 98.5% at concentrations of 50, 75 and 100 µg/ml respectively. Their thrombolytic activities were also observed to increase with concentration and were 59.5, 69.5 and 77.5% for the same concentrations. Appealingly, the AgNPs also showed high antifungal efficacy completely suppressing the mycelial growth of Aspergillus fumigatus, A. niger and A. flavus, at 100 µl/ml with inhibition rates of 93.5, 98.7 and 92.4%, respectively. Furthermore, they demonstrated commendable inhibitory effects against clinical strains in addition to relatively high free radical scavenging activities of 90.96, 88.09 and 85.23% at 50, 75 and 100 µg/ml, respectively. To conclude, AgNPs manufactured by this eco-friendly method have emerged as versatile materials fit for various biomedical applications as advocated by health and innovation Sustainable Development Goals.