Oladipo I.C. , Lateef A., Azeez M.A., Asafa T.B., Yekeen T.A., Ogunsona S.B., Irshad M. & Hakeem A.S.
Vol 2, Issue 1, Article number: 3 (Jul, 2021)
https://doi.org/10.48187/stnanomat.2021.2.001
Highlight
· AuNPs inhibits the mycelia growth of five toxigenic/pathogenic fungi
· AuNPs prevents the coagulation of fresh human blood
· AuNPs rapidly dissolves pre-formed healthy donor human blood clots within 30 min
· AuNPs displays DPPH scavenging activity at concentration dependent rate
Graphical Abstract
Abstract
The green synthesis of gold nanoparticles (AuNps) using crude aqueous extract of Ackee (Blighia sapida) husk as reducing/capping agent was investigated for its antifungal, anticoagulant and thrombolytic activities in this work. The synthesis of AuNps was monitored through the colour change for 15 minutes at photoactivation which turned out to be dark brown colouration and further characterized using UV-Vis spectroscopy, Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The UV-visible spectrum of the AuNPs displayed clear peak at 556.0nm which was not observed in the spectra of the aqueous gold chloride and the crude aqueous extract of the plant. The prominent peaks of AuNps in FTIR spectrum were 3,792.9, 3,346.2, 2,971.4 and 1704.9 cm-1 which lent credence to the fact that proteins and phenolic compounds were involved in the biofabrication and capping of the AuNPs. Energy dispersive X-ray (EDX) analysis showed that gold (Au) was the prominent metal present; the AuNPs were fairly spherical in shape with sizes ranging from 23nm to 126.7nm. The biosynthesized AuNPs showed significant effect againt the growth of Candida albican (38.0%), Aspergillus niger (50%), Aspergillus flavus (63.4%), Aspergilus fumigattus (61.4%) and Fusarium solani (58.9%). The free radical scavenging properties of the AuNps was very potent against the stable organic free radical DPPH, the varying concentration of the AuNPs (10, 20, 40 and 60µg/ml) scavenged DPPH by 80.75, 81.11, 87.91 and 89.34%. Furthermore, the AuNps showed anticoagulant activities of 48.70%, 49.13%, 50.00% and 54.74% at 250µg/ml, 500µg/ml, 750µg/ml and 1000µg/ml and thrombolytic activities of 54.31%, 56.46%, 75.00% and 96.12% at 250µg/ml, 500µg/ml, 750µg/ml and 1000µg/ml. The biomedical and antifungal properties of AuNps against toxigenic/pathogenic fungi established in this research work would grant lasting solution to many medical, agricultural and biotechnological challenges in this dispensation.